Journal: Comparative Hepatology

Article Title: CIDE-A is expressed in liver of old mice and in type 2 diabetic mouse liver exhibiting steatosis

doi: 10.1186/1476-5926-6-4

Figure Lengend Snippet: CIDE-A Northern and Immunoblot analyses . A) Northern analysis of RNA extracted from normal (C) and type 2 diabetic (D) liver and heart tissue. Total RNA (10 μg) from the appropriate tissues was resolved by denaturing agarose gel electrophoresis, transferred to positively charged nylon membrane, hybridized with the [α- 32 P]dCTP-labeled mouse CIDE-A cDNA and exposed to Bio-Max MR film. Ethidium bromide stain of RNA (10 μg/lane) prior to transfer to nylon membrane. The values represent the level of CIDE-A gene expression for the individual tissue as determined by DNA microarray analysis (ND: not determined). The approximated size (1.3 kb) of the CIDE-A mRNA is noted on the right. B) Immunoblot demonstrating increased CIDE-A protein levels in type 2 diabetic mouse liver. Sixty μg of liver and heart extract was electrophoresed on a 12.5% SDS-polyacrylamide gel and the resolved proteins transferred to a nitrocellulose membrane. The membrane was immunoblotted using a rabbit anti-mouse CIDE-A polyclonal antibody and a goat anti-rabbit IgG polyclonal antibody conjugated to horseradish peroxidase. Arrow indicates mouse CIDE-A. The values represent the level of CIDE-A gene expression for the individual tissue as determined by DNA microarray analysis (ND: not determined).

Article Snippet: Resolved proteins were transferred to a nitrocellulose membrane and immunoblotted using a rabbit anti-mouse CIDE-A polyclonal antibody (QED Bioscience Inc., San Diego, CA) as previously described [ ].

Techniques: Northern Blot, Western Blot, Agarose Gel Electrophoresis, Membrane, Labeling, Staining, Gene Expression, Microarray